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BioSpherix pro co2 controller

Figure 1. Hypercapnia limits AT2 cell proliferation in 3D culture organoids. (A) Schematic of experiments designed to coculture AT2 cells isolated from SftpCreERT2 R26REYFP mice (SftpcEYFP AT2) and WT mesenchymal cells. Alveolar organoids were switched to normocapnia (5% <t>CO2;</t> NC) or hypercapnia (20% CO2; HC) media on day 7 and cultured until day 21. (B) Representative images of organoid cultures in normocapnia or hypercapnia. Scale bars: 500 μm. (C) Graph depicts the inhibitory effect of hypercapnia on organoid size. Median with interquartile range. n = 8. (D) Graph depicts the effect of hypercapnia exposure for 21 days on colony forming efficiency (CFE). n = 8. (E) Immunofluorescence analysis of SFTPC (AT2 marker) and Podoplanin (AT1 marker) revealed a reduction in AT2 cell proliferation in organoids exposed to hypercapnia for 14 days relative to normocapnia. Nuclear DNA is stained with DAPI. Scale bars: 50 μm. (C) ANOVA plus Sidak’s multiple comparisons test. (D) Student’s t test. *P < 0.05; ***P < 0.001, ****P < 0.0001.

Pro Co2 Controller, supplied by BioSpherix, used in various techniques. Bioz Stars score: 96/100, based on 415 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Hypercapnia alters stroma-derived Wnt production to limit β-catenin signaling and proliferation in AT2 cells."

Article Title: Hypercapnia alters stroma-derived Wnt production to limit β-catenin signaling and proliferation in AT2 cells.

Journal: JCI insight

doi: 10.1172/jci.insight.159331

Figure Legend Snippet: Figure 1. Hypercapnia limits AT2 cell proliferation in 3D culture organoids. (A) Schematic of experiments designed to coculture AT2 cells isolated from SftpCreERT2 R26REYFP mice (SftpcEYFP AT2) and WT mesenchymal cells. Alveolar organoids were switched to normocapnia (5% CO2; NC) or hypercapnia (20% CO2; HC) media on day 7 and cultured until day 21. (B) Representative images of organoid cultures in normocapnia or hypercapnia. Scale bars: 500 μm. (C) Graph depicts the inhibitory effect of hypercapnia on organoid size. Median with interquartile range. n = 8. (D) Graph depicts the effect of hypercapnia exposure for 21 days on colony forming efficiency (CFE). n = 8. (E) Immunofluorescence analysis of SFTPC (AT2 marker) and Podoplanin (AT1 marker) revealed a reduction in AT2 cell proliferation in organoids exposed to hypercapnia for 14 days relative to normocapnia. Nuclear DNA is stained with DAPI. Scale bars: 50 μm. (C) ANOVA plus Sidak’s multiple comparisons test. (D) Student’s t test. *P < 0.05; ***P < 0.001, ****P < 0.0001.

Techniques Used: Isolation, Cell Culture, Immunofluorescence, Marker, Staining

Figure 2. Transcriptomic analysis of isolated AT2 cells reveals inhibition of βcat signaling during hypercapnia. (A) Hypercapnia decreases the number of cells expressing Ki67 in the alveolar region of the adult mouse lung exposed to room air (RA) or 10% CO2 (HC) for 21 days, as revealed by immunoflu- orescence. White arrows indicate SPTPC+Ki67+ AT2 cells. Scale bars: 20 μm. (B) Graph depicting the inhibitory effect of hypercapnia exposure for 21 days on proliferation. RA, n = 4; HC, n = 3 mice. Student’s t test. **P < 0.01. (C–F) Bulk RNA-Seq was performed on flow cytometry sorted AT2 cells from mice breathing RA (n = 6) or exposed to HC. Heatmap shows clustering of differentially expressed genes (FDR q < 0.05) in AT2 cells after 7 (n = 5) or 21 (n = 5) days of hypercapnia exposure. (D and E) Volcano plots. (F) GO biological processes. (G–J) Expression of selected DEG (FDR q < 0.05) regulated by hypercap- nia involved in the Wnt/βcat pathway.

Figure Legend Snippet: Figure 2. Transcriptomic analysis of isolated AT2 cells reveals inhibition of βcat signaling during hypercapnia. (A) Hypercapnia decreases the number of cells expressing Ki67 in the alveolar region of the adult mouse lung exposed to room air (RA) or 10% CO2 (HC) for 21 days, as revealed by immunoflu- orescence. White arrows indicate SPTPC+Ki67+ AT2 cells. Scale bars: 20 μm. (B) Graph depicting the inhibitory effect of hypercapnia exposure for 21 days on proliferation. RA, n = 4; HC, n = 3 mice. Student’s t test. **P < 0.01. (C–F) Bulk RNA-Seq was performed on flow cytometry sorted AT2 cells from mice breathing RA (n = 6) or exposed to HC. Heatmap shows clustering of differentially expressed genes (FDR q < 0.05) in AT2 cells after 7 (n = 5) or 21 (n = 5) days of hypercapnia exposure. (D and E) Volcano plots. (F) GO biological processes. (G–J) Expression of selected DEG (FDR q < 0.05) regulated by hypercap- nia involved in the Wnt/βcat pathway.

Techniques Used: Isolation, Inhibition, Expressing, RNA Sequencing, Flow Cytometry

Figure 3. Hypercapnia decreases Wnt/βcat signaling in AT2 cells. AT2 cells were isolated from mice exposed to room air (RA) or 10% CO2 (HC) for 21 days. (A) mRNA was isolated, and qPCR was performed. n = 8 mice. (B and C) In situ RNA hybridization showing decreased number of Axin2+ AT2 cells in mice exposed to HC. Yellow arrows indicate Sftpc+Axin2+ AT2 cells. Scale bars: 10 μm. n = 4 mice. (D) Number of lineage-labeled AT2 cells from Axin2CreERT2–TdTom mice determined by flow cytometry. n = 5 mice. Graph shows data from 1 of 3 independent experiments. Student’s t test. *P < 0.05; **P < 0.01.

Figure Legend Snippet: Figure 3. Hypercapnia decreases Wnt/βcat signaling in AT2 cells. AT2 cells were isolated from mice exposed to room air (RA) or 10% CO2 (HC) for 21 days. (A) mRNA was isolated, and qPCR was performed. n = 8 mice. (B and C) In situ RNA hybridization showing decreased number of Axin2+ AT2 cells in mice exposed to HC. Yellow arrows indicate Sftpc+Axin2+ AT2 cells. Scale bars: 10 μm. n = 4 mice. (D) Number of lineage-labeled AT2 cells from Axin2CreERT2–TdTom mice determined by flow cytometry. n = 5 mice. Graph shows data from 1 of 3 independent experiments. Student’s t test. *P < 0.05; **P < 0.01.

Techniques Used: Isolation, In Situ, Hybridization, Labeling, Flow Cytometry

Figure 4. Hypercapnia increases Wnt5a expression in PDGFRα+ fibroblasts. Lung PDGFRα+ fibroblasts were isolated via flow cytometry cell sorting from mice breathing room air (RA) or exposed to 10% CO2 (HC) for 10 days. (A) Expression of Wnt genes in PDGFRα+ fibroblasts as analyzed by population RNA-Seq. n = 3, with cells isolated from 3 mice in each replicate. #FDR q < 0.05). (B–D) mRNA was isolated, and qPCR was performed. (B) Wnt5a (n = 4). (C) Wnt2 (n = 3). (D) MLg2908 mouse lung fibroblast cells were preincubated in the presence or absence of UO126 (10 μM) or PD98059 (10 μM) for 90 minutes and exposed to media equilibrated to NC (5% CO2) or HC (20% CO2) for 24 hours. n = 3. (B and C) Student’s t test. (D) ANOVA plus Sidak’s multiple comparisons test. *P < 0.05; ** P < 0.01.

Figure Legend Snippet: Figure 4. Hypercapnia increases Wnt5a expression in PDGFRα+ fibroblasts. Lung PDGFRα+ fibroblasts were isolated via flow cytometry cell sorting from mice breathing room air (RA) or exposed to 10% CO2 (HC) for 10 days. (A) Expression of Wnt genes in PDGFRα+ fibroblasts as analyzed by population RNA-Seq. n = 3, with cells isolated from 3 mice in each replicate. #FDR q < 0.05). (B–D) mRNA was isolated, and qPCR was performed. (B) Wnt5a (n = 4). (C) Wnt2 (n = 3). (D) MLg2908 mouse lung fibroblast cells were preincubated in the presence or absence of UO126 (10 μM) or PD98059 (10 μM) for 90 minutes and exposed to media equilibrated to NC (5% CO2) or HC (20% CO2) for 24 hours. n = 3. (B and C) Student’s t test. (D) ANOVA plus Sidak’s multiple comparisons test. *P < 0.05; ** P < 0.01.

Techniques Used: Expressing, Isolation, Flow Cytometry, FACS, RNA Sequencing

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Journal: JCI insight

Article Title: Hypercapnia alters stroma-derived Wnt production to limit β-catenin signaling and proliferation in AT2 cells.

doi: 10.1172/jci.insight.159331

Figure Lengend Snippet: Figure 1. Hypercapnia limits AT2 cell proliferation in 3D culture organoids. (A) Schematic of experiments designed to coculture AT2 cells isolated from SftpCreERT2 R26REYFP mice (SftpcEYFP AT2) and WT mesenchymal cells. Alveolar organoids were switched to normocapnia (5% CO2; NC) or hypercapnia (20% CO2; HC) media on day 7 and cultured until day 21. (B) Representative images of organoid cultures in normocapnia or hypercapnia. Scale bars: 500 μm. (C) Graph depicts the inhibitory effect of hypercapnia on organoid size. Median with interquartile range. n = 8. (D) Graph depicts the effect of hypercapnia exposure for 21 days on colony forming efficiency (CFE). n = 8. (E) Immunofluorescence analysis of SFTPC (AT2 marker) and Podoplanin (AT1 marker) revealed a reduction in AT2 cell proliferation in organoids exposed to hypercapnia for 14 days relative to normocapnia. Nuclear DNA is stained with DAPI. Scale bars: 50 μm. (C) ANOVA plus Sidak’s multiple comparisons test. (D) Student’s t test. *P < 0.05; ***P < 0.001, ****P < 0.0001.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 controller (BioSpherix).

Techniques: Isolation, Cell Culture, Immunofluorescence, Marker, Staining

Journal: JCI insight

Article Title: Hypercapnia alters stroma-derived Wnt production to limit β-catenin signaling and proliferation in AT2 cells.

doi: 10.1172/jci.insight.159331

Figure Lengend Snippet: Figure 2. Transcriptomic analysis of isolated AT2 cells reveals inhibition of βcat signaling during hypercapnia. (A) Hypercapnia decreases the number of cells expressing Ki67 in the alveolar region of the adult mouse lung exposed to room air (RA) or 10% CO2 (HC) for 21 days, as revealed by immunoflu- orescence. White arrows indicate SPTPC+Ki67+ AT2 cells. Scale bars: 20 μm. (B) Graph depicting the inhibitory effect of hypercapnia exposure for 21 days on proliferation. RA, n = 4; HC, n = 3 mice. Student’s t test. **P < 0.01. (C–F) Bulk RNA-Seq was performed on flow cytometry sorted AT2 cells from mice breathing RA (n = 6) or exposed to HC. Heatmap shows clustering of differentially expressed genes (FDR q < 0.05) in AT2 cells after 7 (n = 5) or 21 (n = 5) days of hypercapnia exposure. (D and E) Volcano plots. (F) GO biological processes. (G–J) Expression of selected DEG (FDR q < 0.05) regulated by hypercap- nia involved in the Wnt/βcat pathway.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 controller (BioSpherix).

Techniques: Isolation, Inhibition, Expressing, RNA Sequencing, Flow Cytometry

Journal: JCI insight

Article Title: Hypercapnia alters stroma-derived Wnt production to limit β-catenin signaling and proliferation in AT2 cells.

doi: 10.1172/jci.insight.159331

Figure Lengend Snippet: Figure 3. Hypercapnia decreases Wnt/βcat signaling in AT2 cells. AT2 cells were isolated from mice exposed to room air (RA) or 10% CO2 (HC) for 21 days. (A) mRNA was isolated, and qPCR was performed. n = 8 mice. (B and C) In situ RNA hybridization showing decreased number of Axin2+ AT2 cells in mice exposed to HC. Yellow arrows indicate Sftpc+Axin2+ AT2 cells. Scale bars: 10 μm. n = 4 mice. (D) Number of lineage-labeled AT2 cells from Axin2CreERT2–TdTom mice determined by flow cytometry. n = 5 mice. Graph shows data from 1 of 3 independent experiments. Student’s t test. *P < 0.05; **P < 0.01.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 controller (BioSpherix).

Techniques: Isolation, In Situ, Hybridization, Labeling, Flow Cytometry

Journal: JCI insight

Article Title: Hypercapnia alters stroma-derived Wnt production to limit β-catenin signaling and proliferation in AT2 cells.

doi: 10.1172/jci.insight.159331

Figure Lengend Snippet: Figure 4. Hypercapnia increases Wnt5a expression in PDGFRα+ fibroblasts. Lung PDGFRα+ fibroblasts were isolated via flow cytometry cell sorting from mice breathing room air (RA) or exposed to 10% CO2 (HC) for 10 days. (A) Expression of Wnt genes in PDGFRα+ fibroblasts as analyzed by population RNA-Seq. n = 3, with cells isolated from 3 mice in each replicate. #FDR q < 0.05). (B–D) mRNA was isolated, and qPCR was performed. (B) Wnt5a (n = 4). (C) Wnt2 (n = 3). (D) MLg2908 mouse lung fibroblast cells were preincubated in the presence or absence of UO126 (10 μM) or PD98059 (10 μM) for 90 minutes and exposed to media equilibrated to NC (5% CO2) or HC (20% CO2) for 24 hours. n = 3. (B and C) Student’s t test. (D) ANOVA plus Sidak’s multiple comparisons test. *P < 0.05; ** P < 0.01.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 controller (BioSpherix).

Techniques: Expressing, Isolation, Flow Cytometry, FACS, RNA Sequencing

Figure 2. Validation of mouse multi-tissue microarray analysis. (a,b) Validation in mouse tissues. Fzd9, Wnt4, Wnt7a, and Wnt8b expression in the lung (n = 6–7) (a) and diaphragm skeletal muscle (n = 4–5) (b) from mice exposed to normoxic hypercapnia for 7 days. NC, normocapnia; HC, hypercapnia. (c,d) Validation in mouse lung and skeletal muscle cells. Fzd9 and Wnt7a expressions in MLE-12 cells (Ctrl, n = 22–23; 20%CO2, n = 5 per group), ASM cells (Ctrl, n = 22–23; 20%CO2, n = 5 per group) (c), or C2C12 myoblast (Ctrl, n = 18–19; 20%CO2, n = 4–5 per group) or myotube (Ctrl, n = 14–15; 20%CO2, n = 3–4 per group) (d) exposed to high CO2 for up to 24 hours (c) or 6 hours (d). Ctrl, control conditions. All values are represented as mean with error bars shown as the 95% confidence interval. *p < 0.05, **p < 0.01, ***p < 0.001, unpaired two-tailed Student’s t test or one-way ANOVA with Dunnett’s post hoc test.

Journal: Scientific reports

Article Title: Elevated CO 2 regulates the Wnt signaling pathway in mammals, Drosophila melanogaster and Caenorhabditis elegans.

doi: 10.1038/s41598-019-54683-0

Figure Lengend Snippet: Figure 2. Validation of mouse multi-tissue microarray analysis. (a,b) Validation in mouse tissues. Fzd9, Wnt4, Wnt7a, and Wnt8b expression in the lung (n = 6–7) (a) and diaphragm skeletal muscle (n = 4–5) (b) from mice exposed to normoxic hypercapnia for 7 days. NC, normocapnia; HC, hypercapnia. (c,d) Validation in mouse lung and skeletal muscle cells. Fzd9 and Wnt7a expressions in MLE-12 cells (Ctrl, n = 22–23; 20%CO2, n = 5 per group), ASM cells (Ctrl, n = 22–23; 20%CO2, n = 5 per group) (c), or C2C12 myoblast (Ctrl, n = 18–19; 20%CO2, n = 4–5 per group) or myotube (Ctrl, n = 14–15; 20%CO2, n = 3–4 per group) (d) exposed to high CO2 for up to 24 hours (c) or 6 hours (d). Ctrl, control conditions. All values are represented as mean with error bars shown as the 95% confidence interval. *p < 0.05, **p < 0.01, ***p < 0.001, unpaired two-tailed Student’s t test or one-way ANOVA with Dunnett’s post hoc test.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 carbon dioxide controller (BioSpherix).

Techniques: Biomarker Discovery, Microarray, Expressing, Control, Two Tailed Test

Figure 3. Validation of the transcriptomic datasets of hypercapnia in a human bronchial cell line and invertebrates. (a) FZD9 and WNT7a expression in BEAS-2B cells exposed to high CO2 for up to 12 hours (Ctrl, n = 20–23; 20%CO2, n = 5–6 per group). Ctrl, control conditions. (b) Fz and wg expression in Drosophila S2 cells exposed to high CO2 for up to 30 min (Ctrl, n = 14–15; 20%CO2, n = 4–5 per group). All values are represented as mean with error bars shown as the 95% confidence interval. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA with Dunnett’s post hoc test.

Journal: Scientific reports

Article Title: Elevated CO 2 regulates the Wnt signaling pathway in mammals, Drosophila melanogaster and Caenorhabditis elegans.

doi: 10.1038/s41598-019-54683-0

Figure Lengend Snippet: Figure 3. Validation of the transcriptomic datasets of hypercapnia in a human bronchial cell line and invertebrates. (a) FZD9 and WNT7a expression in BEAS-2B cells exposed to high CO2 for up to 12 hours (Ctrl, n = 20–23; 20%CO2, n = 5–6 per group). Ctrl, control conditions. (b) Fz and wg expression in Drosophila S2 cells exposed to high CO2 for up to 30 min (Ctrl, n = 14–15; 20%CO2, n = 4–5 per group). All values are represented as mean with error bars shown as the 95% confidence interval. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA with Dunnett’s post hoc test.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 carbon dioxide controller (BioSpherix).

Techniques: Biomarker Discovery, Expressing, Control

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